Authors (7): M. V. Doble, A. G. Jarvis, A. C. C. Ward, J. D. Colburn, J. P. Götze, M. Bühl, P. C. J. Kamer
Themes: Biocatalysis DOI: 10.1021/acssuschemeng.8b03568
Citations: 27
Pub type: article-journal
Pub year: 2018
Publisher: American Chemical Society (ACS)
Issue: 11
License:
Publication date(s): 2018/11/05 (print) 2018/10/10 (online)
Pages: 15100-15107
Volume: 6 Issue: 11
Journal: ACS Sustainable Chemistry & Engineering
Link: https://pubs.acs.org/doi/pdf/10.1021/acssuschemeng.8b03568
URL: http://dx.doi.org/10.1021/acssuschemeng.8b03568
We report novel artificial metalloenzymes (ArMs), containing tris(pyridylmethyl)amine (TPA), for the atom economic oxidation of lignin β-O-4 model compounds, using hydrogen peroxide. The protein scaffold alters the selectivity of the reaction from a low yielding cleavage reaction when using the parent Fe-tpa complex to a high yielding benzylic alcohol oxidation when using the complex incorporated into a protein scaffold, SCP-2L A100C. Engineering the protein scaffold to incorporate glutamic acid was found to improve the ArM activity, showing that rational design of the protein environment using metal binding amino acids can be a first step toward improving the overall activity of an artificial metalloenzyme.
| Name | Description | Publised |
|---|---|---|
| sc8b03568_si_001.pdf | Supl. data for Artificial Metalloenzymes as Catalysts for Oxidative Lign... | 2018 |
| Computational data for "Artificial Metalloenzymes as Catalysts for Oxidative Lignin Degradation" | Data and metadata for the MD simulations included in the paper\nM. V. Do... | 2018 |
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